Lab 3. Nitrogen Uptake Kinetics

Author

Affiliation

Albertus J. Smit

University of the Western Cape

Published

September 18, 2024

Keywords

Nutrient uptake, Michaelis-Menten kinetics, Algal nutrition

This Lab Accompanies the Following Lecture

Slides: Nutrient Uptake Kinetics
Reading: Michaelis-Menten Uptake Kinetics

Additional Reading

Paper: Smit (2002)

Data For This Lab

The nutrient uptake data – BDC223_Lab_5_Rate calculations.xlsx

Date

Lab Date: 7 October 2024 (Monday)
Due Date: 7:00, 14 October 2024 (Monday)

Students will work as individuals; assignments are per individual. This lab is due on Monday 14 October 2024 at 7:00 on iKamva.

1 Pre-Lab

Read this lab and contextualise within the pertinent material in your text.

2 Post-Lab

Upon completion of this lab:

submit online on Monday 14 October 2024 at 7:00.

3 Task

In this practical, we will practice uptake kinetics calculations that plant biologists interested in nutrient uptake might encounter.

Please read the pertinent theory material in your text and listen to my recorded lectures. The relevant section dealing with the rate calculations is in the PDF slides from under the heading “Uptake kinetics experiments” to the end of “Michaelis-Menten,” but other material in the slides appears elsewhere in the document.

4 Instructions

You may complete the assignment in your own time.

As part of the results presented in a properly formatted MS Word document, I would like to see:

Graphs illustrating the depletion of nutrients over time.
All calculations in the spreadsheet in the columns where ‘???’ is indicated.
$V$ versus $[S]$ plots.
Estimates for $V_{max}$, $K_s$ and $\alpha$
- you can either derive them from the $V$ versus $[S]$ plot
- or, for extra credit, apply the Michaelis-Menten equation and provide parameter estimates along with estimates of their errors).
An abstract that summarises your findings, along with a physiological rationale for these findings.

Ensure that your document is correctly structured (i.e., use headings relevant to the tasks mentioned above and present them in a logical sequence).

Refer to Formatting requirements for all tasks.pdf for further guidance.FOR YOUR ASSIGNMENT, PLEASE SUBMIT YOUR SPREADSHEET. NAME THE FILE AS FOLLOWS: <YOUR_SURNAME>_UPTAKE_RATES.XLS

Reuse

CC BY-NC-SA 4.0

Citation

BibTeX citation:

@online{j._smit2024,
  author = {J. Smit, Albertus},
  title = {Lab 3. {Nitrogen} {Uptake} {Kinetics}},
  date = {2024-09-18},
  url = {http://tangledbank.netlify.app/BDC223/Lab3_nitrogen_uptake.html},
  langid = {en}
}

For attribution, please cite this work as:

J. Smit A (2024) Lab 3. Nitrogen Uptake Kinetics. http://tangledbank.netlify.app/BDC223/Lab3_nitrogen_uptake.html.

--- title: "Lab 3. Nitrogen Uptake Kinetics" date: 2024-09-18 format: html: anchor-sections: true number-sections: true page-layout: article keywords: - Nutrient uptake - Michaelis-Menten kinetics - Algal nutrition --- ::: callout-tip ## This Lab Accompanies the Following Lecture - **Slides:** [Nutrient Uptake Kinetics](../slides/BDC223_uptake_kinetics.pdf) - **Reading:** [Michaelis-Menten Uptake Kinetics](Michaelis-Menten.qmd) ::: ::: callout-tip ## Additional Reading - **Paper:** [Smit (2002)](../docs/Smit_2002.pdf) ::: ::: callout-tip ## Data For This Lab - The nutrient uptake data -- [`BDC223_Lab_5_Rate calculations.xlsx`](../data/BDC223_Lab_5_Rate calculations.xlsx) ::: ::: callout-note ## Date - **Lab Date:** 7 October 2024 (Monday) - **Due Date:** 7:00, 14 October 2024 (Monday) ::: Students will work as individuals; assignments are per individual. [This lab is due on Monday 14 October 2024 at 7:00 on iKamva.]{.my-highlight} # Pre-Lab Read this lab and contextualise within the pertinent material in your text. # Post-Lab Upon completion of this lab: - submit online on Monday 14 October 2024 at 7:00. # Task In this practical, we will practice uptake kinetics calculations that plant biologists interested in nutrient uptake might encounter. Please read the pertinent theory material in your text and listen to my recorded lectures. The relevant section dealing with the rate calculations is in the PDF slides from under the heading “Uptake kinetics experiments” to the end of “Michaelis-Menten,” but other material in the slides appears elsewhere in the document. # Instructions You may complete the assignment in your own time. As part of the results presented in a properly formatted MS Word document, I would like to see: - Graphs illustrating the depletion of nutrients over time. - All calculations in the spreadsheet in the columns where '???' is indicated. - $V$ versus $[S]$ plots. - Estimates for $V_{max}$, $K_s$ and $\alpha$ - you can either derive them from the $V$ versus $[S]$ plot - or, for extra credit, apply the Michaelis-Menten equation and provide parameter estimates along with estimates of their errors). - An abstract that summarises your findings, along with a physiological rationale for these findings. Ensure that your document is correctly structured (i.e., use headings relevant to the tasks mentioned above and present them in a logical sequence). Refer to `Formatting requirements for all tasks.pdf` for further guidance.FOR YOUR ASSIGNMENT, PLEASE SUBMIT YOUR SPREADSHEET. NAME THE FILE AS FOLLOWS: `<YOUR_SURNAME>_UPTAKE_RATES.XLS`